4QF3

Crystal structure of human BAZ2B PHD zinc finger in the free form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-04-28
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.2816
Spacegroup name	P 21 21 21
Unit cell lengths	38.485, 45.605, 65.246
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.830 - 1.600
R-factor	0.19153
Rwork	0.190
R-free	0.22130
Structure solution method	SAD
Starting model (for MR)	Arp/warp autobuilding model
RMSD bond length	0.014
RMSD bond angle	1.660
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	SHELXS
Refinement software	REFMAC (5.7.0029)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.410	29.410	1.700
High resolution limit [Å]	1.600	4.750	1.600
Rmerge	0.029	0.026	0.137
Number of reflections	14800
<I/σ(I)>	29.7	43.86	5.69
Completeness [%]	100.0	100	100
Redundancy	8.4	8.4	8.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.8	293	2.2M Na/K phosphate, pH 7.8, VAPOR DIFFUSION, SITTING DROP, temperature 293K