4QEY
Crystal structure of a DUF4425 family protein (BACOVA_05332) from Bacteroides ovatus ATCC 8483 at 2.52 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-11-26 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 90.590, 101.340, 91.420 |
| Unit cell angles | 90.00, 107.91, 90.00 |
Refinement procedure
| Resolution | 47.347 - 2.520 |
| R-factor | 0.1975 |
| Rwork | 0.196 |
| R-free | 0.22060 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.220 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELX |
| Refinement software | BUSTER-TNT (2.10.0) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 47.347 | 47.347 | 2.610 |
| High resolution limit [Å] | 2.520 | 5.400 | 2.520 |
| Rmerge | 0.078 | 0.045 | 0.744 |
| Number of reflections | 52432 | 5429 | 5126 |
| <I/σ(I)> | 12.67 | 24.4 | 2 |
| Completeness [%] | 98.3 | 98 | 96.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.20M magnesium acetate, 22.0% polyethylene glycol 3350, 3.0% D(+)-sucrose, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
