4QES
Structure of a 16 nm protein cage designed by fusing symmetric oligomeric domains, quadruple mutant, I222 form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-02-28 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9789 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 123.510, 165.520, 167.670 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 83.830 - 4.193 |
R-factor | 0.2535 |
Rwork | 0.251 |
R-free | 0.29480 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.440 |
Data scaling software | XSCALE |
Phasing software | PHASER (2.5.6) |
Refinement software | REFMAC (5.8.0071) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 83.835 | 83.835 | 4.300 |
High resolution limit [Å] | 4.190 | 18.750 | 4.190 |
Rmerge | 0.127 | 0.075 | 0.885 |
Number of reflections | 12914 | 170 | 911 |
<I/σ(I)> | 14.16 | 41.56 | 3.32 |
Completeness [%] | 99.7 | 98.3 | 96.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.4 | 298 | 0.1 M sodium citrate, pH 4.4, 11% PEG3000, 200 mM sodium chloride, VAPOR DIFFUSION, HANGING DROP, temperature 298K |