4QER
Crystal Structure of the Complex of Phospholipase A2 with Resveratrol at 1.20 A Resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2014-02-26 |
Detector | MARRESEARCH |
Wavelength(s) | 0.97 |
Spacegroup name | P 43 |
Unit cell lengths | 52.348, 52.348, 47.246 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.170 - 1.200 |
R-factor | 0.19047 |
Rwork | 0.189 |
R-free | 0.21876 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1fb2 |
RMSD bond length | 0.026 |
RMSD bond angle | 2.213 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.220 |
High resolution limit [Å] | 1.200 | 1.200 |
Rmerge | 0.042 | 0.335 |
Number of reflections | 39365 | |
<I/σ(I)> | 42.2 | 3.8 |
Completeness [%] | 99.1 | 98 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | Ammonium sulphate, PEG 4000 , pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |