4QEQ
High resolution structure of egg white lysozyme
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-06-27 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.976 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 77.650, 77.650, 37.190 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 21.536 - 1.381 |
R-factor | 0.1652 |
Rwork | 0.164 |
R-free | 0.18250 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1dpw |
RMSD bond length | 0.006 |
RMSD bond angle | 1.120 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 24.560 | 1.460 |
High resolution limit [Å] | 1.380 | 1.380 |
Rmerge | 0.048 | 0.350 |
Number of reflections | 23732 | |
<I/σ(I)> | 21 | 5.1 |
Completeness [%] | 80.0 | 100 |
Redundancy | 7.8 | 7.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.9 | 293 | 9% w/v sodium chloride, 0.1 M sodium acetate, pH 4.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K |