4QDY
Crystal structure of a YbbR-like protein (SP_1560) from Streptococcus pneumoniae TIGR4 at 2.74 A resolution
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-07-01 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.91837,0.97966,0.97895 |
Spacegroup name | P 64 2 2 |
Unit cell lengths | 77.930, 77.930, 145.240 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.330 - 2.740 |
R-factor | 0.2346 |
Rwork | 0.232 |
R-free | 0.28780 |
Structure solution method | MAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.070 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHELX |
Refinement software | BUSTER-TNT (2.10.0) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 39.330 | 2.840 | |
High resolution limit [Å] | 2.740 | 5.860 | 2.740 |
Rmerge | 0.139 | 0.013 | 0.013 |
Number of reflections | 7389 | 858 | 727 |
<I/σ(I)> | 18.36 | 45.7 | 2.7 |
Completeness [%] | 99.7 | 98.5 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 42.0% polyethylene glycol 1500, 0.2M ammonium sulfate, 0.1M cesium chloride, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 293K |