4QC2
Crystal structure of lipopolysaccharide transport protein LptB in complex with ATP and Magnesium ions
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 293.25 |
| Detector technology | PIXEL |
| Collection date | 2012-05-10 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.92 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 85.160, 125.760, 89.610 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.810 - 2.220 |
| R-factor | 0.18398 |
| Rwork | 0.181 |
| R-free | 0.24825 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ji0 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.860 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.810 | 2.290 |
| High resolution limit [Å] | 2.200 | 2.220 |
| Rmerge | 0.045 | 0.570 |
| Number of reflections | 24010 | |
| <I/σ(I)> | 1 | 2 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 4.1 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293.25 | 0.2M ammonium fluoride, 20% PEG 3350, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293.25K |
