4QBI
Crystal structure of a stable adenylate kinase variant AKlse6
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-07-21 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.9793 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 36.814, 76.512, 85.130 |
Unit cell angles | 90.00, 90.20, 90.00 |
Refinement procedure
Resolution | 50.000 - 1.670 |
R-factor | 0.17036 |
Rwork | 0.168 |
R-free | 0.20817 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1zin |
RMSD bond length | 0.025 |
RMSD bond angle | 2.428 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.730 |
High resolution limit [Å] | 1.670 | 1.670 |
Number of reflections | 54822 | |
Completeness [%] | 100.0 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 26.5% (w/v) polyethylene glycol 4000, 150mM magnesium acetate, 100mM sodium cacodylate pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |