4QBF

Crystal structure of a stable adenylate kinase variant AKlse2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2013-09-28
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	39.293, 47.202, 109.328
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 1.800
R-factor	0.17382
Rwork	0.171
R-free	0.22247
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4mkg
RMSD bond length	0.019
RMSD bond angle	2.331
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.800
Number of reflections	19418
Completeness [%]	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	1.9M ammonium citrate pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K