4QB7

Crystal structure of a fimbrial protein (BVU_2522) from Bacteroides vulgatus ATCC 8482 at 2.55 A resolution

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL14-1
Synchrotron site	SSRL
Beamline	BL14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-03-06
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.95369,0.97934,0.97919
Spacegroup name	I 2 2 2
Unit cell lengths	54.220, 186.120, 211.830
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.607 - 2.550
R-factor	0.1694
Rwork	0.168
R-free	0.19660
Structure solution method	MAD
RMSD bond length	0.010
RMSD bond angle	1.210
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	BUSTER-TNT (2.10.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.607	46.607	2.640
High resolution limit [Å]	2.550	5.460	2.550
Rmerge	0.087	0.037	0.776
Number of reflections	35435	3730	3459
<I/σ(I)>	12.21	27.7	1.8
Completeness [%]	99.4	96.9	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	277	0.20M sodium chloride, 2.00M ammonium sulfate, 0.1M sodium cacodylate pH 6.5, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K