4QAZ
The crystal structure of the C-terminal domain of Ebola (Zaire) nucleoprotein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-12-06 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97907 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 56.515, 56.515, 63.546 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 26.650 - 1.980 |
R-factor | 0.1859 |
Rwork | 0.184 |
R-free | 0.22910 |
Structure solution method | SAD |
RMSD bond length | 0.012 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.000 | 2.010 |
High resolution limit [Å] | 1.980 | 1.980 |
Number of reflections | 8428 | |
<I/σ(I)> | 12.5 | 3.7 |
Completeness [%] | 97.2 | 76.3 |
Redundancy | 8.7 | 3.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 19.3% PEG3350, 0.3M Magnesium Formate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |