4QAK
Crystal structure of phosphoesterase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 200 |
Detector technology | PIXEL |
Collection date | 2014-04-04 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 4 21 2 |
Unit cell lengths | 107.688, 107.688, 71.657 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.160 - 2.025 |
R-factor | 0.1771 |
Rwork | 0.174 |
R-free | 0.22920 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.106 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHENIX (autosol) |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.160 | 2.100 |
High resolution limit [Å] | 2.020 | 2.020 |
Number of reflections | 27221 | |
Completeness [%] | 97.2 | 83.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 295 | 2M NaCl, 0.1mM sodium acetate, pH 5, VAPOR DIFFUSION, SITTING DROP, temperature 295K |