4QAJ
Crystal structure of Peptidyl-tRNA hydrolase from Pseudomonas aeruginosa at 1.5 Angstrom resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2013-11-02 |
Detector | MARRESEARCH |
Wavelength(s) | 0.97 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 64.115, 64.115, 155.312 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.640 - 1.500 |
R-factor | 0.18206 |
Rwork | 0.180 |
R-free | 0.22027 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4jc4 |
RMSD bond length | 0.021 |
RMSD bond angle | 1.926 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.640 | 1.530 |
High resolution limit [Å] | 1.500 | 1.500 |
Number of reflections | 31169 | |
<I/σ(I)> | 50.3 | 2 |
Completeness [%] | 99.8 | 99.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | PEG 4000, 5% isoproponal, 100mM HEPES buffer, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |