4Q9X
mTFP* PdCl2 soak
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-09-07 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 34.470, 83.630, 37.820 |
| Unit cell angles | 90.00, 112.01, 90.00 |
Refinement procedure
| Resolution | 15.000 - 1.900 |
| R-factor | 0.17849 |
| Rwork | 0.176 |
| R-free | 0.21653 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hqk |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.267 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.036 | 0.586 |
| Number of reflections | 15454 | |
| <I/σ(I)> | 16.5 | 1.9 |
| Completeness [%] | 98.2 | 98.3 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 25.9% PEG3000 100mM MES, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
