4Q9R
Crystal structure of an RNA aptamer bound to trifluoroethyl-ligand analog in complex with Fab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2014-04-07 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 147.515, 78.986, 94.357 |
| Unit cell angles | 90.00, 112.42, 90.00 |
Refinement procedure
| Resolution | 67.680 - 3.120 |
| R-factor | 0.2056 |
| Rwork | 0.203 |
| R-free | 0.25310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.545 |
| Data reduction software | RAPD |
| Data scaling software | RAPD |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.9_1678)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.680 | 3.290 |
| High resolution limit [Å] | 3.120 | 3.120 |
| Number of reflections | 17701 | |
| Completeness [%] | 98.1 | 98.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 8% Tacsimate pH 7.0, 20% PEG 3,350, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
