4Q9Q
Crystal structure of an RNA aptamer bound to bromo-ligand analog in complex with Fab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2014-04-05 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9184 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 146.545, 79.676, 95.280 |
| Unit cell angles | 90.00, 111.51, 90.00 |
Refinement procedure
| Resolution | 46.430 - 2.450 |
| R-factor | 0.1837 |
| Rwork | 0.182 |
| R-free | 0.22050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.695 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.9_1678)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.430 | 2.480 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.151 | 0.589 |
| Number of reflections | 72602 | |
| <I/σ(I)> | 12.8 | 0.8 |
| Completeness [%] | 98.5 | 95.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.1 | 298 | 8% Tacsimate pH 7.0, 16% PEG 3,350, 0.1M cacodylate pH 7.1, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
