4Q9G

Crystal structure of K12V/C16S/C117V/P134V mutant of human acidic fibroblast growth factor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2013-11-14
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.020
Spacegroup name	C 2 2 21
Unit cell lengths	75.395, 97.327, 108.198
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.180 - 1.554
R-factor	0.171
Rwork	0.170
R-free	0.19930
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.020
RMSD bond angle	1.761
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX ((Phaser MR))
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.610
High resolution limit [Å]	1.550	1.550
Number of reflections	55669
Completeness [%]	97.0	90.5
Redundancy		11.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	1.8 M sodium formate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K