4Q95

Crystal structure of HRASLS3/LRAT chimeric protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	77
Detector technology	PIXEL
Collection date	2013-07-30
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9795
Spacegroup name	P 43 21 2
Unit cell lengths	63.123, 63.123, 157.965
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.320 - 2.200
R-factor	0.20922
Rwork	0.207
R-free	0.24767
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4dot
RMSD bond length	0.014
RMSD bond angle	1.676
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall
Low resolution limit [Å]	49.320
High resolution limit [Å]	2.200
Number of reflections	16797
Completeness [%]	98.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	298	0.1 M Tris/HCl, pH 8.5, 0.2 M NaCl, 20% (w/v) polyethylene glycol 3350, VAPOR DIFFUSION, SITTING DROP, temperature 298K