4Q8H

Structure of the Saccharomyces cerevisiae PAN2 pseudoubiquitin-hydrolase-RNase module

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2013-07-30
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.939080
Spacegroup name	I 2 2 2
Unit cell lengths	91.712, 115.505, 257.449
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.013 - 3.100
R-factor	0.2406
Rwork	0.239
R-free	0.27530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 1WLJ AND 4Q8G
RMSD bond length	0.004
RMSD bond angle	0.872
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	AutoSol
Refinement software	PHENIX ((phenix.refine: dev_1647))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.013	3.320
High resolution limit [Å]	3.100	3.100
Rmerge	0.133	2.488
Number of reflections	25287
<I/σ(I)>	9.6	0.7
Completeness [%]	100.0	100
Redundancy	5.3	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	100 mM sodium acetate, pH 5.5, 10% v/v PEG200, VAPOR DIFFUSION, SITTING DROP, temperature 293K