4Q8G

Structure of the Saccharomyces cerevisiae PAN2 pseudoubiquitin-hydrolase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-02-25
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.999970
Spacegroup name	P 21 21 21
Unit cell lengths	68.508, 74.032, 175.081
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.328 - 2.100
R-factor	0.1857
Rwork	0.184
R-free	0.22090
Structure solution method	SAD
RMSD bond length	0.014
RMSD bond angle	1.411
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	AutoSol
Refinement software	PHENIX ((phenix.refine: dev_1631))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.160
High resolution limit [Å]	2.100	2.100
Rmerge	0.082	1.210
Number of reflections	52731
<I/σ(I)>	10.2	0.8
Completeness [%]	99.8	99.7
Redundancy	5.2	5.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	230 mM tri-ammonium citrate, pH 7.0, 16% w/v PEG3350, VAPOR DIFFUSION, SITTING DROP, temperature 293K