4Q8G
Structure of the Saccharomyces cerevisiae PAN2 pseudoubiquitin-hydrolase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-02-25 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.999970 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 68.508, 74.032, 175.081 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.328 - 2.100 |
| R-factor | 0.1857 |
| Rwork | 0.184 |
| R-free | 0.22090 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.411 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | AutoSol |
| Refinement software | PHENIX ((phenix.refine: dev_1631)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.160 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.082 | 1.210 |
| Number of reflections | 52731 | |
| <I/σ(I)> | 10.2 | 0.8 |
| Completeness [%] | 99.8 | 99.7 |
| Redundancy | 5.2 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 230 mM tri-ammonium citrate, pH 7.0, 16% w/v PEG3350, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
