4Q8E
Human DNA polymerase eta inserting dCMPNPP opposite a phenanthriplatin adducted G
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-01 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 61 |
| Unit cell lengths | 98.766, 98.766, 82.197 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 26.937 - 1.549 |
| R-factor | 0.1864 |
| Rwork | 0.183 |
| R-free | 0.22530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 1.580 |
| High resolution limit [Å] | 1.549 | 4.200 | 1.550 |
| Rmerge | 0.078 | 0.036 | 0.660 |
| Number of reflections | 65955 | ||
| <I/σ(I)> | 10.2 | ||
| Completeness [%] | 99.8 | 97.9 | 99.5 |
| Redundancy | 3.4 | 3.3 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | PEG 2000-MME, MES, pH 6.0, vapor diffusion, hanging drop, temperature 293K |
