4Q86
YcaO with AMP Bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-08-18 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97872 |
| Spacegroup name | P 1 |
| Unit cell lengths | 111.254, 112.949, 132.886 |
| Unit cell angles | 89.94, 73.51, 77.23 |
Refinement procedure
| Resolution | 127.140 - 2.250 |
| R-factor | 0.21937 |
| Rwork | 0.218 |
| R-free | 0.24999 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.481 |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 127.140 | 2.260 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Number of reflections | 264918 | |
| <I/σ(I)> | 10 | 2 |
| Completeness [%] | 93.1 | 66.1 |
| Redundancy | 3.8 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 18% PEG 8,000 0.1 M magnesium acetate 0.1 M sodium cacodylate pH 6.5, 8 mg/mL protein, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
