4Q7Q
The crystal structure of a possible lipase from Chitinophaga pinensis DSM 2588
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-03-10 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97924 |
Spacegroup name | P 43 |
Unit cell lengths | 80.611, 80.611, 93.005 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.200 - 1.451 |
R-factor | 0.155 |
Rwork | 0.153 |
R-free | 0.18970 |
Structure solution method | SAD |
RMSD bond length | 0.005 |
RMSD bond angle | 0.988 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | SHELXD |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.200 | 1.480 |
High resolution limit [Å] | 1.450 | 1.450 |
Rmerge | 0.091 | 0.628 |
Number of reflections | 104383 | |
<I/σ(I)> | 46.3 | 3.6 |
Completeness [%] | 99.9 | 100 |
Redundancy | 9.7 | 9.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 289 | 0.1M CHES:NaOH, 30% (v/v) PEG 400, pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |