4Q7N
Crystal structure of the complex of Buffalo Signalling protein SPB-40 with 4-N-trimethylaminobutyraldehyde at 1.79 Angstrom Resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 77 |
Detector technology | CCD |
Collection date | 2013-11-02 |
Detector | MARRESEARCH |
Wavelength(s) | 0.97 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 61.068, 66.735, 106.072 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 56.490 - 1.790 |
R-factor | 0.18698 |
Rwork | 0.185 |
R-free | 0.22941 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2o9o |
RMSD bond length | 0.027 |
RMSD bond angle | 2.458 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 56.500 | |
High resolution limit [Å] | 1.790 | 1.790 |
Number of reflections | 40939 | |
<I/σ(I)> | 27.7 | 2.1 |
Completeness [%] | 99.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 298 | pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 298K |