4Q7M
Structure of NBD288-Avi of TM287/288
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 90 |
Detector technology | PIXEL |
Collection date | 2012-09-14 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9999 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 58.300, 58.300, 208.690 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.489 - 2.300 |
R-factor | 0.1834 |
Rwork | 0.181 |
R-free | 0.23600 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 0.944 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.9_1678)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.489 |
High resolution limit [Å] | 2.300 |
Number of reflections | 19132 |
Completeness [%] | 99.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293.15 | 100 mM Sodium acetate, 6 % PEG550 MME, 6 % PEG20000, 200 mM MgCl2, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 293.15K |