4Q7L
Structure of NBD288 of TM287/288
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 90 |
Detector technology | PIXEL |
Collection date | 2013-05-28 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.000 |
Spacegroup name | P 32 |
Unit cell lengths | 95.930, 95.930, 88.510 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.965 - 2.350 |
R-factor | 0.1952 |
Rwork | 0.193 |
R-free | 0.23630 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 0.853 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.9_1678)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 47.965 |
High resolution limit [Å] | 2.350 |
Number of reflections | 37661 |
Completeness [%] | 99.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293.15 | 100 mM Tris-HAOAc pH 7.5, 200 mM MgCl2, 8 % PEG550 MME, 8 % PEG20000, VAPOR DIFFUSION, SITTING DROP, temperature 293.15K |