4Q79
Structure of a HG-derivative CsgG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-03-13 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.006 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 171.640, 176.880, 104.990 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.247 - 3.100 |
| R-factor | 0.2381 |
| Rwork | 0.236 |
| R-free | 0.27830 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.207 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Number of reflections | 66194 | |
| Completeness [%] | 60.0 | 59.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 50mM Tris-HCl (pH 8.5), 300mM MgCl2, 14% PEG2000, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
