4Q77
Crystal structure of Rot, a global regulator of virulence genes in Staphylococcus aureus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-03-17 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 31.747, 71.505, 65.680 |
| Unit cell angles | 90.00, 99.38, 90.00 |
Refinement procedure
| Resolution | 32.420 - 1.770 |
| R-factor | 0.16588 |
| Rwork | 0.163 |
| R-free | 0.21720 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.089 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.770 |
| Number of reflections | 27813 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.6 | 285 | 1.5M Ammonium chloride, 0.1M Sodium acetate trihydrate PH 4.6, VAPOR DIFFUSION, temperature 285K |
