4Q76
Crystal structure of Nfs2 C384S mutant, the plastidial cysteine desulfurase from Arabidopsis thaliana
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-12-16 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.980111 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 81.718, 68.387, 87.580 |
| Unit cell angles | 90.00, 94.84, 90.00 |
Refinement procedure
| Resolution | 40.713 - 1.900 |
| R-factor | 0.2346 |
| Rwork | 0.233 |
| R-free | 0.26160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1t3i |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.853 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.020 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.374 | |
| Number of reflections | 75702 | |
| <I/σ(I)> | 3.1 | |
| Completeness [%] | 99.7 | 99.4 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | microbatch under oil | 7.5 | 278 | 20% PEG3000, 0.2M sodium chloride and 0.1M HEPES pH 7.5, microbatch under oil , temperature 278K |
