4Q75
Crystal structure of Nfs2, the plastidial cysteine desulfurase from Arabidopsis thaliana
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-12 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.978542 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 81.567, 68.205, 87.066 |
| Unit cell angles | 90.00, 94.76, 90.00 |
Refinement procedure
| Resolution | 45.853 - 1.709 |
| R-factor | 0.204 |
| Rwork | 0.203 |
| R-free | 0.23260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1t3i |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.127 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.853 | 1.800 |
| High resolution limit [Å] | 1.709 | 1.710 |
| Rmerge | 0.187 | |
| Number of reflections | 97823 | |
| <I/σ(I)> | 3.5 | |
| Completeness [%] | 95.0 | 75 |
| Redundancy | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | microbatch under oil | 7.5 | 278 | 20% PEG3000, 0.2M sodium chloride and 0.1M HEPES pH 7.5, microbatch under oil, temperature 278K |
