4Q74

F241A Fc

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Detector technology	PIXEL
Collection date	2012-01-27
Detector	DECTRIS PILATUS 6M
Spacegroup name	P 21 21 21
Unit cell lengths	57.910, 74.700, 113.710
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.650 - 2.190
Rwork	0.206
R-free	0.23200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1h3x
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (refine)

Data quality characteristics

	Overall
Low resolution limit [Å]	35.650
High resolution limit [Å]	2.190
Number of reflections	44637

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	30% PEG 1500 at 20 C and cryopreserved in well solution containing 25% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 293.15K