4Q70

Light Harvesting Protein Phycocyanin in high resolution using a femtosecond X-Ray laser

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	FREE ELECTRON LASER
Source details	SLAC LCLS BEAMLINE CXI
Synchrotron site	SLAC LCLS
Beamline	CXI
Temperature [K]	283
Detector technology	PIXEL
Collection date	2014-01-25
Detector	Cornell-SLAC Pixel Array Detector (CSPAD)
Wavelength(s)	1.34
Spacegroup name	H 3 2
Unit cell lengths	192.800, 192.800, 62.500
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.436 - 1.850
R-factor	0.2079
Rwork	0.204
R-free	0.27460
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	1.425
Data reduction software	CrystFEL
Data scaling software	CrystFEL
Phasing software	PHASER
Refinement software	PHENIX (1.9_1675)

Data quality characteristics

	Overall
Low resolution limit [Å]	36.436
High resolution limit [Å]	1.850
Number of reflections	37798
Completeness [%]	100.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Batch	7	277	10-18 % PEG 3350, 10-100 mM NaCl, 0-100 mM MgCl2, pH 7.0, Batch, temperature 277K