4Q6T
The crystal structure of a class V chitininase from Pseudomonas fluorescens Pf-5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-12-13 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 52.022, 57.327, 110.797 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.458 - 1.400 |
| R-factor | 0.1375 |
| Rwork | 0.136 |
| R-free | 0.17180 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.079 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXS |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.500 | 1.420 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.092 | 0.714 |
| Number of reflections | 65837 | |
| <I/σ(I)> | 40 | 2.81 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.1 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 297 | 0.005 M Cobalt Chloride, 0.005 M Magnesium Chloride, 0.005 M Cadmium Chloride, 0.005 M Nickel Chloride, 0.1 M HEPES:NaOH, 12% (w/v) PEG 3350, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 297K |
