4Q6F
Crystal structure of human BAZ2A PHD zinc finger in complex with unmodified H3K4 histone peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-01-18 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97626 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 71.866, 71.866, 98.767 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.060 - 1.910 |
R-factor | 0.18472 |
Rwork | 0.183 |
R-free | 0.21864 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.012 |
RMSD bond angle | 1.553 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 29.060 |
High resolution limit [Å] | 1.910 |
Rmerge | 0.062 |
Number of reflections | 20562 |
<I/σ(I)> | 20.4 |
Completeness [%] | 100.0 |
Redundancy | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 2M malic acid, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 277K |