4Q65

Structure of the E. coli Peptide Transporter YbgH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	CCD
Collection date	2012-04-12
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9788
Spacegroup name	P 21 21 2
Unit cell lengths	94.035, 109.448, 76.659
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.540 - 3.400
R-factor	0.2603
Rwork	0.258
R-free	0.31570
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	1.273
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELXS
Refinement software	PHENIX ((phenix.refine: 1.8_1069))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.700
High resolution limit [Å]	3.400	7.100	3.400
Number of reflections	9105
Completeness [%]	72.0	88.4	45.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	293	50mM NaAc (pH 5.0), 25%(v/v) PEG400, 50mM Mg(Ac)2, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	5	293	50mM NaAc (pH 5.0), 25%(v/v) PEG400, 50mM Mg(Ac)2, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	5	293	50mM NaAc (pH 5.0), 25%(v/v) PEG400, 50mM Mg(Ac)2, VAPOR DIFFUSION, HANGING DROP, temperature 293K