4Q63
Crystal Structure of Legionella Uncharacterized Protein Lpg0364
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-07-14 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97931 |
| Spacegroup name | P 65 |
| Unit cell lengths | 82.117, 82.117, 38.689 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.158 - 1.953 |
| R-factor | 0.185 |
| Rwork | 0.183 |
| R-free | 0.22100 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.016 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((phenix.refine: 1.8.1_1161)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.980 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Number of reflections | 10988 | |
| <I/σ(I)> | 11.5 | 2.2 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 11 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 0.05M Cd Chloride, 0.1M HEPES pH7.5, 15%PEG400, 11%Etylen Glycol, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
