4Q5I
Crystal structure of a T151A mutant of the E. coli FeoB G-domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-04-05 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.03000 |
Spacegroup name | P 1 |
Unit cell lengths | 60.978, 82.779, 95.505 |
Unit cell angles | 115.67, 108.50, 90.06 |
Refinement procedure
Resolution | 45.060 - 2.800 |
R-factor | 0.2415 |
Rwork | 0.239 |
R-free | 0.29829 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 0.682 |
Data reduction software | MOSFLM |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.900 |
High resolution limit [Å] | 2.800 | 6.030 | 2.800 |
Rmerge | 0.066 | 0.032 | 0.367 |
Number of reflections | 45132 | ||
<I/σ(I)> | 11.2 | ||
Completeness [%] | 97.2 | 97.9 | 96.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 293 | 29% PEG 400, 0.1M HEPES pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |