4Q5G
Crystal Structure of mouse Serum Amyloid A3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-07-31 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9794 |
Spacegroup name | P 62 |
Unit cell lengths | 78.327, 78.327, 62.319 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 39.163 - 2.057 |
R-factor | 0.1712 |
Rwork | 0.167 |
R-free | 0.21350 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.900 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHENIX |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.050 |
Number of reflections | 12206 |
Completeness [%] | 97.0 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293 | 75-80% MPD, 0.1M sodium acetate , pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |