4Q5G
Crystal Structure of mouse Serum Amyloid A3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-07-31 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 62 |
| Unit cell lengths | 78.327, 78.327, 62.319 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.163 - 2.057 |
| R-factor | 0.1712 |
| Rwork | 0.167 |
| R-free | 0.21350 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.900 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.050 |
| Number of reflections | 12206 |
| Completeness [%] | 97.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293 | 75-80% MPD, 0.1M sodium acetate , pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
