4Q58
Crystal structure of the plectin 1a actin-binding domain/integrin beta 4 fragment complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 0.873 |
| Spacegroup name | P 65 |
| Unit cell lengths | 96.341, 96.341, 208.025 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 46.929 - 4.001 |
| R-factor | 0.2268 |
| Rwork | 0.224 |
| R-free | 0.28690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.892 |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.160 | 4.470 |
| High resolution limit [Å] | 4.000 | 4.000 |
| Rmerge | 0.272 | 0.793 |
| Number of reflections | 9188 | |
| <I/σ(I)> | 7.4 | 3.9 |
| Redundancy | 5.7 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 20 mM HEPES, pH 6.5, 150 mM sodium formate, 7.5% PEG550 MME, 3% sucrose, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
