4Q51
Crystal structure of a putative molybdenum cofactor biosynthesis protein F from Burkholderia cenocepacia J2315
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-02-27 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97872 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.402, 46.163, 77.293 |
| Unit cell angles | 90.00, 99.14, 90.00 |
Refinement procedure
| Resolution | 29.570 - 1.900 |
| R-factor | 0.16274 |
| Rwork | 0.160 |
| R-free | 0.20871 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.789 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.930 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.080 | 0.500 |
| Number of reflections | 37347 | |
| <I/σ(I)> | 24.8 | 4.2 |
| Completeness [%] | 96.7 | 93.7 |
| Redundancy | 6.6 | 6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 292 | 0.2 M Calcium Chloride, 0.1 M Tris-HCl, 25 % (w/v) PEG4000, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 292K |
