4Q4J

Structure of crosslinked TM287/288_S498C_S520C mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	90
Detector technology	PIXEL
Collection date	2013-05-28
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.99987
Spacegroup name	C 1 2 1
Unit cell lengths	216.670, 84.140, 113.460
Unit cell angles	90.00, 93.32, 90.00

Refinement procedure

Resolution	48.464 - 3.200
R-factor	0.2391
Rwork	0.237
R-free	0.28680
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.201
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	3.200
Number of reflections	33360
Completeness [%]	98.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	293.15	32 % PEG 400, 50 mM Na-Acetate pH 5 and 500 mM KCl, 2.5 mM AMP-PNP, 3 mM MgCl2, VAPOR DIFFUSION, SITTING DROP, temperature 293.15K