4Q48
Structure of the RecQ Catalytic Core from Deinococcus radiodurans
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE BL13C1 |
Synchrotron site | NSRRC |
Beamline | BL13C1 |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 2012-10-30 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9762 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 85.791, 98.528, 152.880 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.312 - 2.797 |
R-factor | 0.218 |
Rwork | 0.213 |
R-free | 0.28570 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1oyw |
RMSD bond length | 0.011 |
RMSD bond angle | 1.577 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.900 |
High resolution limit [Å] | 2.797 | 2.800 |
Rmerge | 0.543 | |
Number of reflections | 31511 | |
Completeness [%] | 96.3 | 96.6 |
Redundancy | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.7 | 277 | 0.1M HEPES (pH 7.7), 14% PEG 8K, 8% ethylene glycol, VAPOR DIFFUSION, SITTING DROP, temperature 277K |