4Q46
The second structure of Influenza B PB2 cap-binding domain complex with GDP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-04-07 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97917 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 43.074, 88.543, 52.151 |
Unit cell angles | 90.00, 105.18, 90.00 |
Refinement procedure
Resolution | 24.570 - 1.800 |
R-factor | 0.17301 |
Rwork | 0.170 |
R-free | 0.23065 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4oro |
RMSD bond length | 0.025 |
RMSD bond angle | 2.170 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 24.570 | 1.830 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.077 | 0.217 |
Number of reflections | 33162 | |
Completeness [%] | 94.6 | 98.2 |
Redundancy | 5.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20% PEG 3350, 0.2M Sodium formate, VAPOR DIFFUSION, SITTING DROP, temperature 293K |