4Q3O
Crystal structure of MGS-MT1, an alpha/beta hydrolase enzyme from a Lake Matapan deep-sea metagenome library
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-06-10 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 189.454, 131.397, 112.441 |
| Unit cell angles | 90.00, 103.55, 90.00 |
Refinement procedure
| Resolution | 19.720 - 1.740 |
| R-factor | 0.1472 |
| Rwork | 0.145 |
| R-free | 0.18520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3fak |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.482 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.9pre_1669)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.720 | 1.830 |
| High resolution limit [Å] | 1.740 | 1.740 |
| Rmerge | 0.056 | 0.392 |
| Number of reflections | 272067 | |
| <I/σ(I)> | 21.3 | 2.2 |
| Redundancy | 3 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 0.1M MES, 20% PEG10K. Cryroprotectant: 12% Glycerol then paraton-N oil, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
