4Q3J

Crystal structure of NFkB-p65-degrading zinc protease family protein

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-10-06
Detector	ADSC QUANTUM 4
Wavelength(s)	1.2574,1.2831,1.2835
Spacegroup name	P 21 21 21
Unit cell lengths	45.205, 67.480, 81.688
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.942 - 1.862
R-factor	0.1833
Rwork	0.179
R-free	0.21810
Structure solution method	MAD
RMSD bond length	0.014
RMSD bond angle	0.993
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (1.7.1_743)
Refinement software	PHENIX ((phenix.refine: 1.7.1_743))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.862
Number of reflections	19840
Completeness [%]	96.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	289	10% PEG-3350, 50 mM Mg(CHO2)2, 0.1 M MES pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K