4Q3I
Structure of the OsSERK2 leucine rich repeat extracellular domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-09-19 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 67.881, 50.646, 83.161 |
Unit cell angles | 90.00, 113.25, 90.00 |
Refinement procedure
Resolution | 42.215 - 2.350 |
R-factor | 0.2411 |
Rwork | 0.236 |
R-free | 0.28580 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.612 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX (dev_1525) |
Refinement software | PHENIX ((phenix.refine: dev_1616)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.390 |
High resolution limit [Å] | 2.350 | 2.350 |
Number of reflections | 22466 | |
Completeness [%] | 95.0 | 71.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 100 mM Bis-Tris pH 6.5, 200 mM MgCl2, and 23% (w/v) PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 298K |