4Q37
Crystal structure of the hypothetical protein TM0182 Thermotoga maritima, N-terminal domain.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2010-08-08 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0698 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 102.050, 145.300, 141.710 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.600 - 3.190 |
R-factor | 0.2375 |
Rwork | 0.235 |
R-free | 0.28000 |
Structure solution method | SAD |
RMSD bond length | 0.004 |
RMSD bond angle | 0.936 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | SHELX |
Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 39.600 | 4.500 | 3.260 |
High resolution limit [Å] | 3.190 | 4.300 | 3.190 |
Rmerge | 0.014 | ||
Number of reflections | 33708 | ||
<I/σ(I)> | 15.01 | ||
Completeness [%] | 99.1 | 100 | 88.5 |
Redundancy | 6.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1 M HEPES, 1.2 M Ammonium sulfate and 0.3 M NaCl, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |