4Q35
Structure of a membrane protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-03-13 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97906 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 240.823, 116.620, 68.038 |
| Unit cell angles | 90.00, 103.71, 90.00 |
Refinement procedure
| Resolution | 40.628 - 2.393 |
| R-factor | 0.1886 |
| Rwork | 0.186 |
| R-free | 0.23070 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.256 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASES |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.590 |
| High resolution limit [Å] | 2.393 | 5.170 | 2.400 |
| Rmerge | 0.124 | ||
| Number of reflections | 71148 | ||
| Completeness [%] | 99.7 | 99.6 | 99.6 |
| Redundancy | 12 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 289 | 10 mM sodium citrate (pH 5.6), 300mM lithium sulfate, 8% PEG 400, 100mM glycine, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
