4Q2X

Crystal structure of Arginyl-tRNA synthetase complexed with L-canavanine

Experimental procedure

Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Detector technology	CCD
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.98703
Spacegroup name	P 21 21 21
Unit cell lengths	75.393, 109.310, 175.011
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.476 - 2.798
R-factor	0.2144
Rwork	0.212
R-free	0.26520
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.186
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.850
High resolution limit [Å]	2.798	7.560	2.800
Rmerge	0.103	0.064	0.313
Number of reflections	35623
<I/σ(I)>	10
Completeness [%]	97.8	97.5	95.7
Redundancy	5.3	7.9	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.7	293	0.085mM Sodium citrate tribasic dehydrate, 24% Polyethylene glycol 4000, 0.17M Ammonium acetate, 15% Glycerol, pH 5.7, VAPOR DIFFUSION, HANGING DROP, temperature 293K