4Q2U

Crystal structure of the E. coli DinJ-YafQ toxin-antitoxin complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2011-11-07
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97
Spacegroup name	C 1 2 1
Unit cell lengths	176.540, 120.920, 120.830
Unit cell angles	90.00, 130.77, 90.00

Refinement procedure

Resolution	43.906 - 1.800
R-factor	0.1785
Rwork	0.178
R-free	0.21460
Structure solution method	SAD
RMSD bond length	0.015
RMSD bond angle	1.424
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHELXD
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.820
High resolution limit [Å]	1.760	1.760
Number of reflections	353442
Completeness [%]	98.9	94.41
Redundancy	1.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	1.9-2.3M Ammonium Sulfate, VAPOR DIFFUSION, SITTING DROP, temperature 277.0K